Which of the following modifications listed below is NOT required to express a eukaryotic gene inside of a bacterium?

Which of the following modifications listed below is NOT required to express a eukaryotic gene inside of a bacterium?
A. Concerting DNA into cDNA
B. Replacement of eukaryotic termination sequences with prokaryotic sequences
C. Making sure coding sequence is cloned downstream of prokaryotic promoter
D. Addition of poly-A tail